In an alternate timeline first appearing in the "Spider-Verse" crossover comic, "Edge of Spider-Verse" #2...
...'Gwen Stacy' is bitten by a radioactive spider, becoming superhero 'Spider-Woman' aka 'Spider-Gwen':
"...she’s smart, she’s tough, she’s charming and she can lift a car – she’s 'Gwen Stacy'! Patrolling the New York City of her universe, she keeps the streets safe as the vigilante known as 'Spider-Woman'! Just don’t tell her father, 'NYPD Police Chief George Stacy'..."
Variations of 'Spider-Man' in the new film could include 'Ben Reilly' aka 'Scarlet Spider', 'Mac Gargan', possessing the 'Venom' symbiote...
...'Spider-X' aka 'Brian Kornfield', a demonic Spider-Man, 'Mattie Franklin', niece of 'J. Jonah Jameson', granted spider powers by 'The Gathering of Five'...
...'Ai Apaec', the South American spider god, transformed into a six armed version of Spider-Man, the Spidey robot 'Timespinner', the clones 'Kaine' and 'Spidercide'...
...the evil 'Blood Spider' and 'Spider Doppelganger', mystical Ezekiel Sims', member of the 'Spider Society' and 'WebCorps'.
And last but not least, fan favorite 'Peter Porker', 'The Amazing Spider-Ham'.
Click the images to enlarge...